Ethidium Bromide Resistance, a New Marker on the Staphylococcal Penicillinase Plasmid
نویسندگان
چکیده
منابع مشابه
Ethidium bromide resistance, a new marker on the staphylococcal penicillinase plasmid.
A strain of Staphylococcus aureus resistant to ethidium bromide is described. The genetic determinants for the resistance are present on the same plasmid as the penicillinase genes.
متن کاملEffects of ethidium bromide on growth and on loss of the penicillinase plasmid of Staphylococcus aureus.
Ethidium bromide (EB) was more efficient than ethyl violet or rifampin as a curing agent for the penicillinase plasmids of Staphylococcus aureus strains. The effects of EB on growth and on the loss of the penicillinase plasmid of PS 81 were studied in detail. The growth rates of PS 81 and an EB-cured derivative were identical in broth, but the cured derivative had a shorter lag in the presence ...
متن کاملMechanism of Ethidium Bromide Fluorescence Enhancement on
The mechanism of the enhancement of the fluorescence of ethidium bromide on binding to double helical RNA and DNA has been investigated. From an examination of the effect of different solvents on the fluorescence lifetime, quenching of fluorescence by proton acceptors, and the substantial lengthening of lifetime observed upon deuteration of the amino protons, regardless of the medium, we conclu...
متن کاملIn Vivo Studies on Staphylococcal Penicillinase.
Eyckmans, Luc (Baylor University Medical Center, Dallas, Tex.), and Ralph Tompsett. In vivo studies on staphylococcal penicillinase. J. Bacteriol. 90:589-593. 1965.-The amounts of staphylococcal penicillinase present in tissue of mice with well-established infections due to penicillin-resistant staphylococci were determined. The data indicate that, in the presence of such infection, penicillina...
متن کاملLocalization of the major ethidium bromide binding site on tRNA.
Binding of ethidium bromide to Escherichia coli tRNAVal and an RNA minihelix based on the acceptor stem and T-arm of tRNAVal was investigated by 19F and 1H NMR spectroscopy of RNAs labeled with fluorine by incorporation of 5-fluorouracil. Ethidium bromide selectively intercalates into the acceptor stem of the tRNAVal. More than one ethidium bromide binding site is found in the acceptor stem, th...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1969
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.100.3.1413-1414.1969